化学
核酸
检出限
荧光
胶体金
线性范围
氯霉素
DNA
酶
免疫分析
滚动圆复制
组合化学
分子生物学
生物物理学
色谱法
生物化学
纳米颗粒
抗体
纳米技术
聚合酶
抗生素
免疫学
生物
材料科学
物理
量子力学
作者
Weiwei Shen,Hong Qing,Hao Xiang,Yijing Zhou,Yahui Guo,Zhenmin Liu
出处
期刊:Analytical Sciences
[Japan Society for Analytical Chemistry]
日期:2024-04-12
标识
DOI:10.1007/s44211-024-00565-z
摘要
A fluorescent immunosorbent assay incorporating signal amplification away from the surface of spherical nucleic acid (SNA) was developed for the detection of chloramphenicol (CAP). Through the conjugation of antibodies and poly-adenine (polyA) DNA onto the surface of gold nanoparticles (AuNPs), the fabrication of the nano-immunoprobe was achieved in a more straightforward and cost-effective manner. Moreover, a strategy utilizing the hybridization chain reaction (HCR) in the amplification step was devised, with particular attention given to the enzyme inhibition associated with SNA. The results demonstrated good performance on CAP detection with a linear range of 0.01–5 ng/L with a detection limit of 0.005 ng/L. The significance of this work mainly lies in the polyA-SNA-based immunoprobe and the thoughtful design to prevent enzyme inhibition.
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