中性粒细胞胞外陷阱
NADPH氧化酶
先天免疫系统
流式细胞术
化学
自噬
细胞外
细胞生物学
髓过氧化物酶
PI3K/AKT/mTOR通路
MAPK/ERK通路
生物化学
活性氧
炎症
生物
细胞凋亡
分子生物学
信号转导
免疫学
受体
作者
Lanqiu Zhang,Zexun Hu,Lei Yang,Tianyu Li,Jing Xun,Qi Zhang,Ximo Wang,Hejun Gao,Zhenjing Jin
标识
DOI:10.1080/14786419.2024.2343918
摘要
This study aimed to investigate the effects of SSa, one of the major triterpenoid saponins extracted from Radix bupleuri, on neutrophil extracellular trap (NET) formation and the mechanism associated with this process. Using Sytox green and immunofluorescence assays, we found SSa rapidly induced NET formation, which depended on NADPH oxidase (NOX)-independent ROS production and autophagy. Pharmacologic inhibitor studies indicated that ERK and PI3K/AKT signalling were also required for SSa-induced NET formation, whereas protein arginine deiminase 4 (PAD4) was not required. Furthermore, we found that SSa promoted neutrophil bactericidal activity mainly through NET formation. Based on flow cytometry and the Cell Counting Kit-8 (CCK-8) assays, the results demonstrated that SSa-induced NET formation occurred without neutrophil death. Taken together, these findings indicated that SSa could be a potential natural product to boost innate immune defense against pathogen attack via NET formation.
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