化学
过氧化氢
试剂
超氧化物
活性氧
一氧化氮
选择性
羟基自由基
水溶液
细胞内
光化学
氧气
组合化学
激进的
有机化学
生物化学
催化作用
酶
作者
Michelle C. Y. Chang,Arnd Pralle,Ehud Y. Isacoff,Christopher J. Chang
摘要
We present the synthesis, properties, and biological applications of Peroxyfluor-1 (PF1), a new type of optical probe for intracellular imaging of hydrogen peroxide in living biological samples. PF1 utilizes a boronate deprotection mechanism to provide unprecedented selectivity and optical dynamic range for detecting H2O2 in aqueous solution over similar reactive oxygen species including superoxide, nitric oxide, tert-butyl hydroperoxide, and hydroxyl radical. We further demonstrate the value of this reagent for biological applications by imaging changes in [H2O2] in living mammalian cells.
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