生物
细胞毒性T细胞
CD8型
T细胞
白细胞介素21
细胞因子
细胞生物学
抗原
肿瘤坏死因子α
分子生物学
细胞凋亡
抗原提呈细胞
免疫学
体外
免疫系统
生物化学
作者
Martin Pelletier,Alessandra Micheletti,Marco A. Cassatella
摘要
Abstract CD8+ T cells modulate neutrophil apoptosis and surface marker expression via TNF-α, IFN-γ and GM-CSF, while CD4+ T cells effects involve IFN-γ and GM-CSF. Neutrophils and T cells often co-infiltrate pathological tissues, which suggests that these 2 cell types may interact with each other. Over the years, in vitro studies have demonstrated that neutrophils and T cells are capable of modulating each otherˈs responses. However, few studies have examined the cross-talk between human neutrophils and CD8+ T cells, although murine models clearly show the critical role of their interaction in bacterial infections and cancer. Herein, we evaluated the interaction between human neutrophils and CD8+ T cells in a coculture system using highly purified cell preparations and compared the responses to ones from cocultures of neutrophils and CD4+ T cells. We report that anti-CD3-activated CD4+ and more potently, anti-CD3-activated CD8+ T cells modulate apoptosis and expression of activation markers by neutrophils, and neutrophils have no or little effect on T cell survival, expression of surface markers, and cytokine release. The observed effects of CD8+ T cells on neutrophils were mainly attributable to the release of TNF-α, IFN-γ, and GM-CSF, and the effects of CD4+ T cells involved IFN-γ and GM-CSF production. This study expands our knowledge of the molecular bases, whereby human CD4+ and CD8+ T cells modulate neutrophil survival and antigen expression.
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