造血
脐带
骨保护素
祖细胞
外周血单个核细胞
化学
体外
间质细胞
骨桥蛋白
间充质干细胞
帘布衬里
离体
免疫学
干细胞
男科
脐带血
医学
细胞生物学
胎盘脐带储备
骨髓
内科学
病理
生物
生物化学
基因
激活剂(遗传学)
作者
Elena R. Andreeva,M. I. Ezdakova,P. I. Bobyleva,Irina V. Andrianova,Andrey Ratushnyy,Ludmila Buravkova
标识
DOI:10.1007/s10517-021-05266-5
摘要
The effectiveness of stroma-dependent expansion of hematopoietic cells ex vivo may depend on the level of commitment of multipotent mesenchymal stromal cells (MSC). Markers of MSC osteodifferentiation and the level of soluble hematopoiesis regulators were determined during their interaction with umbilical cord blood mononuclears. After 72-h co-culturing, an increase in the expression of ALPL and alkaline phosphatase activity was revealed. In conditioned medium of co-cultures, the levels of osteopontin and osteoprotegerin were elevated and the levels of osteocalcin and sclerostin were reduced. Co-culturing of umbilical cord blood mononuclears with osteocommitted MSC was accompanied by more pronounced increase in the concentration of both positive (GM-CSF and G-CSF) and negative (IP-10, MIP-1α, and MCP-3) regulators of hematopoiesis. Thus, umbilical cord blood mononuclears induced the formation of early osteogenic progenitor phenotype in MSC ex vivo, providing the microenvironmental conditions necessary to support hematopoiesis. Preliminary osteocommitted MSC were more sensitive to the effect of umbilical cord blood mononuclears.
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