锤头状核酶
核酶
环状RNA
发夹状核酶
哺乳动物CPEB3核酶
核糖核酸
核酶
连接酶核酶
分子生物学
生物
内含子
抄写(语言学)
作者
Akinori Ochi,So Umekage,Yo Kikuchi
出处
期刊:Nucleic Acids Symposium Series
[Oxford University Press]
日期:2009-09-01
卷期号:53 (53): 275-276
被引量:6
摘要
Circular RNA is more stable than linear RNA both in vitro and in vivo because of its inaccessibility to exoribonucleases. Therefore, circularization of functional RNAs is a potentially useful methodology for designing therapeutic RNA reagents. We designed a circular hammerhead ribozyme that can cleave the template region of human telomerase RNA. This circular hammerhead ribozyme was generated by in vitro transcription followed by spontaneous self-circularization activity using the permuted intron-exon (PIE) method. Two-dimensional gel electrophoresis and alkaline digestion of the in vitro transcription products revealed that the circular hammerhead ribozyme could be produced by the PIE method. The purified circular hammerhead ribozyme cleaved the template region of human telomerase RNA in a magnesium-dependent manner. These results indicated that the circular hammerhead ribozyme generated by the PIE method maintained the specific feature of canonical hammerhead catalytic activity.
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