胶粘剂
抗菌活性
化学
体内
蛋白质吸附
细胞粘附
金黄色葡萄球菌
粘附
吸附
酶
细胞
材料科学
细菌
微生物学
纳米技术
生物物理学
生物化学
生物
生物技术
有机化学
图层(电子)
遗传学
作者
Jianshu Li,Chunmei Ding,Mingzhen Wu,Xinyuan Xu,Xiang Ke,Hao Xu,Juan Li,Feng Lou,Kai Zhou,Haolun Jiang,Peng Xu,Xianlong Wang,Longlong Si,Jianshu Li
标识
DOI:10.1016/j.cej.2021.128955
摘要
With the long-term clinical study of implantation materials, the problem of bioinertness on the implant surface emerged. The bioinert implant surface can cause inflammation, fibrosis, coagulation, and infections, and even threaten the life of patients. Herein, we designed and synthesized a saliva-acquired pellicle (SAP)-inspired multifunctional polypeptide DDDEEKRWRWRWGPLGVRGD (SAP-MP196-G-1) by orderly assembling the hydroxyapatite (HA)-adsorbing motif DDDEEK, antibacterial motif RWRWRW, bacterial enzyme-responding motif GPLGV, and cell adhesion motif RGD. The bioinspired polypeptide can self-assemble into nanoparticles and tightly adsorb onto the biomineral, such as the HA surface. The newly formed bio-interface exhibited two-fold higher adhesion and proliferation of MG63 cells and five-fold higher antibacterial properties against Staphylococcus aureus (S. aureus) compared to bare HA group. An in vivo study indicated that the bio-interface can promote implant wound healing and decrease infection of S. aureus on HA-coated implants. This study proposes a strategy to design a cell-adhesive and antibacterial-biomineral interface based on a dynamic enzyme-digestion reaction to effectively inhibit the side effects of implantation surface presence.
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