免疫分析
生物标志物
医学
抗体
前列腺癌
前列腺特异性抗原
内科学
癌症
抗原
分子生物学
接收机工作特性
癌症研究
作者
Yuan-Hung Pong,Yann-Rong Su,Hsing-Wen Lo,Chung-Kun Ho,Chia-Chi Hsieh,Ching-Tung Chu,Yui Whei Chen-Yang,Vincent F.S. Tsai,Jui-Chuang Wu,Guang-Jer Wu
摘要
Background METCAM/MUC18 expression was increased with the malignant progression of prostate cancer and also a bona fide metastatic gene, capable of initiating and driving the metastasis of a non-metastatic human prostate cancer cell line to multiple organs. Objective We explored if METCAM/MUC18 was detectable in human serum and a novel biomarker to predict malignant propensity of prostate cancer. Materials and methods Two antibodies were identified by Western blot analysis having the highest sensitivity and specificity to establish calibration curves from the recombinant METCAM/MUC18 proteins. They were used in ELISA and LFIA to determine the METCAM/MUC18 concentrations in serum samples from 8 normal individuals, 4 BPH patients, 1 with PIN, 6 with high-grade prostate cancer, and 2 treated cancer patients. Results Serum METCAM/MUC18 concentrations were statistically significantly higher in the patients with PIN and prostate cancer than those with BPH, the treated patients and normal individuals. The LFIA results were statistically better than ELISA and Western blot methods. Serum METCAM/MUC18 concentrations were in direct proportional to most of serum PSA concentrations.
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