甲基化
生物
N6-甲基腺苷
热应力
基因
DNA甲基化
信使核糖核酸
编码区
分子生物学
遗传学
基因表达
甲基转移酶
动物科学
作者
Zengkui Lu,Jianbin Liu,Chao Yuan,Meilin Jin,Kai Quan,Mingxing Chu,Caihong Wei
出处
期刊:Genomics
[Elsevier]
日期:2021-01-01
卷期号:113 (1): 484-492
被引量:16
标识
DOI:10.1016/j.ygeno.2020.09.038
摘要
N6-methyladenosine (m6A) mRNA methylation varies in response to stress. However, no map of m6A mRNA methylation has been obtained for sheep, nor is it known what effect this has on regulating heat stress in sheep. Here, we obtained m6A methylation maps of sheep liver tissues with and without heat stress by MeRIP-seq. In total, 8306 m6A peaks associated with 2697 genes were detected in the heat stress group, and 12,958 m6A peaks associated with 5494 genes were detected in the control group. Peaks were mainly enriched in coding regions and near stop codons with classical RRACH motifs. Methylation levels of heat stress and control sheep were higher near stop codons, although methylation was significantly lower in heat stress sheep. GO and KEGG revealed that differential m6A-containing genes were significantly enriched in the stress response and fat metabolism. Our results showed that m6A mRNA methylation modifications regulate heat stress in sheep.
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