基质凝胶
细胞外基质
基底膜
干细胞
基质(化学分析)
细胞生物学
层粘连蛋白
细胞培养
生物
细胞
化学
生物化学
色谱法
遗传学
作者
Chris Hughes,Lynne-Marie Postovit,Gilles Lajoie
出处
期刊:Proteomics
[Wiley]
日期:2010-04-28
卷期号:10 (9): 1886-1890
被引量:1150
标识
DOI:10.1002/pmic.200900758
摘要
Abstract Numerous cell types require a surface for attachment to grow and proliferate. Certain cells, particularly primary and stem cells, necessitate the use of specialized growth matrices along with specific culture media conditions to maintain the cells in an undifferentiated state. A gelatinous protein mixture derived from mouse tumor cells and commercialized as Matrigel is commonly used as a basement membrane matrix for stem cells because it retains the stem cells in an undifferentiated state. However, Matrigel is not a well‐defined matrix, and therefore can produce a source of variability in experimental results. In this study, we present an in‐depth proteomic analysis of Matrigel using a dynamic iterative exclusion method coupled with fractionation protocols that involve ammonium sulfate precipitation, size exclusion chromatography, and one‐dimensional SDS‐PAGE. The ability to identify the low mass and abundance components of Matrigel illustrates the utility of this method for the analysis of the extracellular matrix, as well as the complexity of the matrix itself.
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