萜类
萜烯
倍半萜
化学
生物合成
碳氢化合物
细胞色素P450
立体化学
组合化学
有机化学
酶
作者
Man-Cheng Tang,Cheng Shen,Zixin Deng,M. Ohashi,Yi Tang
出处
期刊:Organic Letters
[American Chemical Society]
日期:2022-06-23
卷期号:24 (26): 4783-4787
被引量:3
标识
DOI:10.1021/acs.orglett.2c01785
摘要
Terpenoids are an important class of natural products with diverse structures and bioactivities. Their hydrocarbon scaffolds are mainly derived from the terpenes produced by terpene cyclases (TCs). Otherwise, new hydrocarbon scaffolds can be achieved through oxidative rearrangement catalyzed by oxygenases such as P450s. Herein, we report the functional characterization of α/β-trans-bergamotene-producing TCs and their multifunctional P450 partners mined from different fungal species. In addition, novel sesquiterpenoids with hydrocarbon scaffolds different from bergamotenes were generated by combinatorial biosynthesis through mixing-and-matching these TC and P450 pairs. Our results provide a successful example of expanding the chemical diversity of terpenoids by combining genome mining and synthetic biology.
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