免疫分析
等离子体子
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
2019年冠状病毒病(COVID-19)
2019-20冠状病毒爆发
纳米技术
材料科学
病毒学
光电子学
医学
传染病(医学专业)
抗体
疾病
病理
爆发
免疫学
作者
Sungwoo Lee,Seonghyeon Lee,Woongkyu Park,Soohyun Lee,Soon Wook Kwon,Myeong Jin Oh,MohammadNavid Haddadnezhad,Insub Jung,Bohyeon Kim,Jieun Park,Kuk Hyun Shin,Hyungdon Lee,Jayeon Yoo,Won Keun Kim,Sungho Park
出处
期刊:Nano Letters
[American Chemical Society]
日期:2024-04-01
标识
DOI:10.1021/acs.nanolett.4c00451
摘要
This study represents the synthesis of a novel class of nanoparticles denoted as annular Au nanotrenches (AANTs). AANTs are engineered to possess embedded, narrow circular nanogaps with dimensions of approximately 1 nm, facilitating near-field focusing for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via a surface-enhanced Raman scattering (SERS)-based immunoassay. Notably, AANTs exhibited an exceedingly low limit of detection (LOD) of 1 fg/mL for SARS-CoV-2 spike glycoproteins, surpassing the commercially available enzyme-linked immunosorbent assay (ELISA) by 6 orders of magnitude (1 ng/mL from ELISA). To assess the real-world applicability, a study was conducted on 50 clinical samples using an SERS-based immunoassay with AANTs. The results revealed a sensitivity of 96% and a selectivity of 100%, demonstrating the significantly enhanced sensing capabilities of the proposed approach in comparison to ELISA and commercial lateral flow assay kits.
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