Design of Non-Thermal Plasma Alfalfa Seed Vigor Enhancement Device and Study of Treatment Effect

Highlights An effective seed treatment method is provided. Three generations of field growth trials were conducted. We Investigated the effects of low-temperature plasma treatment on the biological characters and yield components. Abstract . An atmospheric pressure, low-temperature dielectric barrier discharge (DBD) plasma seed treatment device was developed for plasma seed treatment. The device worked continuously on alfalfa seeds and evenly distributed the seeds in a plasma discharge range. The processing time, voltage amplitude, and frequency were adjustable. The device was used to study the effect of DBD plasma treatment at different voltages and times on alfalfa seed germination using untreated alfalfa seeds as the control (CK). The results showed that the DBD plasma treatment of alfalfa seeds promoted seed germination and seedling growth, and the optimal discharge conditions were a discharge voltage of 11 kV and a discharge time of 40 s. Compared with CK, the germination potential and germination rate increased by 12.49% and 18.08%, respectively. After treatment using the optimal discharge time, the germination potential, germination rate, dry weight, and seedling height increased by 9.9%, 16.1%, 15%, and 32.9%, respectively, compared with CK. The Scanning Electron Microscope images of the seed epidermis treated with 11 kV and 40 s plasma showed that the surface of alfalfa seeds was etched. Different doses of discharge radiation had different effects on physiological processes in seeds, and their sensitivity to plasma discharge was different. In a certain range, the germination rate, germination potential, fresh weight, dry weight, root length, and seedling height of alfalfa seeds improved to different degrees under different discharge voltages and times. Plasma has a good application prospect for improving the growth of alfalfa seeds. Keywords: Alfalfa, Dielectric barrier discharge plasma, Germination, Seed treatment device, Seedling growth.