Lacticaseibacillus salsurae sp. nov. and Levilactobacillus muriae sp. nov., isolated from traditional Chinese pickle

Two novel bacteria (designated N501-2 T and N40-8-2 T ) belonging to the genera Lacticaseibacillus and Levilactobacillus were isolated from traditional Chinese pickle (‘Suan cai’) and identified. Strain N501-2 T was phylogenetically related to the type strains of Lacticaseibacillus baoqingensis , Lacticaseibacillus porcinae , Lacticaseibacillus manihotivorans and Lacticaseibacillus jixiensis , having 98.7–99.4% 16S rRNA gene sequence similarities. Strain N40-8-2 T was phylogenetically related to the type strains of Levilactobacillus fuyuanensis , Levilactobacillus parabrevis , Levilactobacillus tujiorum , Levilactobacillus hammesii , Levilactobacillus senmaizukei and Levilactobacillus tangyuanensis , having 98.4–99.4% 16S rRNA gene sequence similarities. Strain N501-2 T had 74.0–84.2% ANI (average nucleotide identity), 20.8–28.4% dDDH (digital DNA–DNA hybridization) and 73.5–86.8% AAI (average amino acid identity) values with L. manihotivorans DSM 13343 T , L. porcinae JCM 19617 T , L. jixiensis N163-3-2 T and L. baoqingensis 47-3 T . Analyses based on whole-genome sequences indicated that strain N40-8-2 T was most closely related to the type strains of L. fuyuanensis , L. parabrevis , L. tujiorum and L. hammesii , having less than 87.4% ANI, 33.6% dDDH and 93.2% AAI values. Based upon the data obtained in the present study, two novel species, Lacticaseibacillus salsurae sp. nov. and Levilactobacillus muriae sp. nov., are proposed, and the type strains are N501-2 T (=CCTCC AB 2024124 T =JCM 37150 T =LMG 33663 T ) and N40-8-2 T (=CCTCC AB 2024128 T =JCM 37001 T =LMG 33659 T ), respectively.