MOF-based hybrid electrodes for multi-enzyme cascade reactions with stabilized mediator immobilization

ZIF67-NQS/CNT hybrid electrodes enabling stable mediator immobilization and efficient multi-enzyme cascade reactions exhibited enhanced current output attributed to the cascade effect. Notably, the pyruvate byproduct inhibited lactate oxidase activity, while its elimination via pyruvate decarboxylase significantly improved the current response. This strategy offers both mechanistic insights and practical advantages.