A Novel Glucose Electrochemical Sensor Based on Au‐Pt Bimetallic Hydrogel Operating in Neutral Media

Abstract A novel nonenzymatic glucose sensor has been developed using Au‐Pt bimetallic hydrogels as the sensing material, synthesized via a facile reduction method. The prepared materials were characterized for their morphology and composition using scanning electron microscopy (SEM), energy‐dispersive X‐ray spectroscopy (EDS), and X‐ray diffraction (XRD). Electrochemical responses of the hydrogels to glucose were investigated using cyclic voltammetry (CV) and chronoamperometry in phosphate buffer (PB) solution at pH 7.4. Among various Au‐Pt ratios tested, the Au‐Pt BMH with a molar ratio of 1:2 exhibited the highest catalytic performance towards glucose oxidation, showing a sensitive response within the concentration range of 1–20 mM ( R 2 = 0.9946) and a limit of detection (LOD) of 31.88 µM. Moreover, this sensor demonstrated excellent selectivity against common physiological interferents and maintained high stability over multiple tests and long‐term storage at −4 °C. Importantly, the sensor also showed a reliable linear response to additional glucose concentrations up to 5 mM in serum samples ( R 2 = 0.9918), indicating its potential for practical clinical applications. This study highlights the promising application of Au‐Pt BMH in developing efficient, reliable, nonenzymatic electrochemical sensors for glucose monitoring under neutral pH conditions, contributing significantly to diabetes management and related metabolic disorder diagnostics.