枯草芽孢杆菌
电穿孔
转化(遗传学)
质粒
重组DNA
生物
拉伤
微生物学
计算生物学
化学
分子生物学
DNA
生物化学
细菌
遗传学
基因
解剖
作者
Tien Thi My Pham,Trang Thi Phuong Phan
出处
期刊:Tạp chí Phát triển Khoa học Công nghệ
[Viet Nam National University Ho Chi Minh City]
日期:2015-06-30
卷期号:18 (2): 16-24
标识
DOI:10.32508/stdj.v18i2.1139
摘要
Bacillus subtilis has been developed as an attractive expression host because of many advantages. For examples, it is nonpathogenic and allows secretion of functional extracellular proteins directly into the culture medium; about 60 % of industrial enzymes available produced by Bacillus species. To use B. subtilis strain for research and as host strain for expression of recombinant protein, bacterial genetic methods should be developed. Electroporation to transfer directly DNA into B. subtilis is one of the methods that draw a lot of attention of scientists. A problem encountered in the methods that draw a lot of attention of scientists. A problem encountered in the electroporation of DNA into B. subtilis is that an established protocol for one strain can hardly be used for another strain. B. subtilis 1012 and WB800N have recently been used as expression hosts for expression of recombinant proteins, but electroporation method has not been established. In this study, we use a pHT plasmid to establish an electroporation protocol for B. subtilis 1012 and WB800N. The influence of sampling time, concentration and time for incubating with lysozyme, voltage on the transformation was investigated to establish the protocol.
科研通智能强力驱动
Strongly Powered by AbleSci AI