基因传递
转染
核酸
三元络合物
共价键
三元运算
纳米技术
化学
遗传增强
阳离子聚合
基因
计算生物学
材料科学
生物
生物化学
计算机科学
酶
有机化学
程序设计语言
作者
Shuai Liu,Tianying Guo
出处
期刊:Current Drug Metabolism
[Bentham Science]
日期:2015-07-13
卷期号:16 (2): 152-165
被引量:6
标识
DOI:10.2174/138920021602150713115608
摘要
Recent progress in gene therapy has opened the door for various human diseases. The greatest challenge that gene vectors still face is the ability to sufficiently deliver nucleic acid into target cells. To overcome various barriers, plenty of researches have been undertaken utilizing diverse strategies, among which a wide variety of polycation/pDNA vectors have been developed and explored frequently. For enhanced transfection efficiency, polycations are constantly utilized with covalent modifications, which however lead to reduced positive charge density and changed properties of polycation/pDNA complexes. Accordingly, non-covalent or ternary strategy is proposed. The cationic properties of polycations can be retained and the transfection efficiency can be enhanced by introducing additional polymers with functional groups via non-covalent assembly. This review will discuss the construction and advantages of ternary complexes gene delivery system, including low toxicity and enhanced gene expression both in vitro and in vivo. Recent progress and expectations with promising results that may have some reference for clinical application are also discussed. Keywords: Gene vectors, non-covalent, polycations, ternary complexes, transfection.
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