染色质
空间组织
分区(防火)
生物
折叠(DSP实现)
染色体
基因组组织
核小体
染色体构象捕获
基因组
进化生物学
功能(生物学)
遗传学
计算生物学
着丝粒
相间
基因
增强子
电气工程
工程类
基因表达
酶
生物化学
作者
Siyuan Wang,Jessica Su,Brian J. Beliveau,Bogdan Bintu,Jeffrey R. Moffitt,Chao-ting Wu,Xiaowei Zhuang
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2016-08-05
卷期号:353 (6299): 598-602
被引量:530
标识
DOI:10.1126/science.aaf8084
摘要
The spatial organization of chromatin critically affects genome function. Recent chromosome-conformation-capture studies have revealed topologically associating domains (TADs) as a conserved feature of chromatin organization, but how TADs are spatially organized in individual chromosomes remains unknown. Here, we developed an imaging method for mapping the spatial positions of numerous genomic regions along individual chromosomes and traced the positions of TADs in human interphase autosomes and X chromosomes. We observed that chromosome folding deviates from the ideal fractal-globule model at large length scales and that TADs are largely organized into two compartments spatially arranged in a polarized manner in individual chromosomes. Active and inactive X chromosomes adopt different folding and compartmentalization configurations. These results suggest that the spatial organization of chromatin domains can change in response to regulation.
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