Effects of paclitaxel on the quantitative proliferation and migration of rabbit vascular smooth muscle cells and endothelial cells

Objective To investigate the effects of paclitaxel on the quantitative proliferation and migration of rabbit vascular smooth muscle cells (SMC) and endothelial cells (EC). Methods In the cell coculture system, rabbit EC were seeded on the lower chamber and SMC on the upper chamber. 3H-TdR incorporation and proliferating cell nuclear antigen ( PCNA ) protein expression were used and migration rate was analyzed to determine the effects of paclitaxel on the migration of rabbit vascular EC and SMC. The IC50 of paclitaxel on EC and SMC was calculated. Results The 3H-TdR incorporation, PCNA protein expression and cell migration rate of SMC were inhibited by paclitaxel between 1 nmol/L and 1 μmol/L in a concentration-dependent manner( n = 6, P 0. 05 ) ; those of EC were also inhibited by paclitaxel between 10 nmol/L and 1μmol/L in a concentration-dependent manner (n =6, P 0. 05) ; the 3H-TdR incorporation and PCNA protein expression of EC were not inhibited by paclitaxel of 1 nmol/L, but the migration of EC was inhibited significantly by paclitaxel of 1 nmol/L (n = 6, P 0. 05 ) . The IC50 of paclitaxel on the proliferation and migration of rabbit vascular SMC was (10. 09±0. 47) nmol/L and (9. 16±0. 54) nmol/L respectively. The IC50 of paclitaxel on the proliferation and migration of rabbit vascular EC respectively were (19. 05 ±0. 35) nmol/L and (5. 37 ±0. 51) nmol/L. Conclusion Paclitaxel could inhibit the proliferation and migration of rabbit vascular SMC and EC , delay EC regeneration in a concentration-dependent manner.