Chemical constituents from root of Lasianthus acuminatissimus I

Objective To investigate the chemical constituents from the root of Lasianthus acuminatissimus of Rubiaceae. Method Normal, reverse phase silica gel, macroporous resin column and HPLC chromatography were used for isolation. Spectroscopic methods (13C-NMR, 1H-NMR, DEPT, EI-MS) were used for identification. Result Six compounds were isolated and elucidated as isoscopletin(I), isoscopletin-6-O-beta-D-glucopyranoside (II), beta configuration of Mudanoside-A (Ill), asperuloside (IV), (-)-pinoresinol 4-O-beta-D-glucopyranoside (V), daucosterol (VI). Conclusion These compounds were obtained from this plant for the first time.