Distribution of SBA receptors and microstructure of spermatogenic cells during spermatogenesis of Atractomorpha sinensis

The microstructure of spermatogenic cells during spermatogenesis of Atractomorpha sinensis Bolivar was observed by the microscopic methods,and distribution of the soybean agglutinin (SBA) receptors during spermatogenesis was detected by using lectin-binding cytochemistry method. The results demonstrated that the process of spermatogenesis in A. sinensis could be divided into 5 stages,including the spermospore proliferation stage,the primary spermatocyte stage,the secondary spermatocyte stage,the spermateliosis stage and the spermiotiliosis stage. During the whole process,great changes took place in size,morphology and chromosomes of the spermatogenic cells. The results of SBA labeling showed that the GalNAc glycoconjugates first appeared at spermospore proliferation stage,and experienced obvious modification during the spermatocyte stage,but at the spermateliosis and the spermiotiliosis stage,the GalNAc glycoconjugates disappeared. These results suggested that during the spermatogenesis of A. sinensis,the quantity and location of the GalNAc glycoconjugates apparently changed,which may be responsible for the growth and differentiation of the spermatogenetic cells.