Ultra-Performance Liquid Chromatography for Quantification of Flavone in Astragalus membranaceus Preparation

Objective:To establish UPLC method for the determination of main flavone in Astragalus membranaceus peparation.Method: ACQUITY UPLCTMBEH C18column(2.1 mm×50 mm,1.7 μm) was used with Methanol-0.2% formic acid Wateras a mobile phase.The flow rate was 0.5 mL·min-1,The detecting wavelength was set at 200-400 nm.The column temperature was at 30 ℃.Result: The linear ranges of calycosin glycoside,formononetin glycoside,calycosin and formononetin were 0.131 3-1.313 g·L-1(r=0.999 7),0.118 6-1.186 g·L-1(r=0.999 4),0.020 6-0.206 g·L-1(r=0.999 5),0.0150-0.150 g·L-1(r=0.999 5).The average recoveries were 97.07%,97.26%,97.45%,96.97%.Conclusion: The method was reliable,accurate and quickly.It can be used for quality control of A.membranaceus peparation.