Studies on the Interaction Between Geranylgeranyl Pyrophosphate Synthase and Phytoene Synthase of Erwinia Uredovora

Geranylgeranyl pyrophosphate synthase(GGPP synthase) and phytoene synthase are two key enzymes in synthesis metabolism of carotenoids in Erwinia uredovora.In this study,crtE encoding GGPP synthase and crtB encoding phytoene synthase were co-transformed into E.coliBL21(DE3) to construct an engineering bacterium.Meanwhile,crtE,crtB as well as crtI encoding phytoene desaturase were also transformed into E.coli BL21(DE3) to construct another engineering bacterium.After induced by IPTG,expressed recombinant proteins were purified by affinity chromatography and gel filtration chromatography.GGPP synthase and phytoene synthase were found to have formed two complexes,while phytoene desaturase showed no interaction with these two enzymes.