Type 2 rat diabetic models induced by high-fat feeding combined with Streptozocin

Objective To study type 2 rat diabetic models induced by high-fat feeding combined with STZ.Method Five to six weeks old SD rats were injected with low doses of STZ after being fed with high-fat and high-sugar diets for 4 weeks.One week thereafter their blood fasting glucose(FBG),triglyceride(TG),total cholesterol(T-CHO),and serum insulin(INS) levels were measured;rats with FBG higher than 7.0 mmol/L were classified as diabetic models.The models were then subjected to continuous monitoring of body weight,water consumption and urine collection,OGTT,TG,T-CHO,and INS.These models' effectiveness was checked by continuous doses of Metformin Hydrochloride(200 mg/kg),Rosiglitazone Maleate(3,6,12 mg/kg),Glimepiride(2,4,8 mg/kg) for 5 d,which were typical treatments for type 2 diabets.Results Group B(Low dose group,with high fatsugar diets and 30 mg/kg STZ) did not develop diabetic symptoms and FBG was lower than 7.0 mmol/L;while group C(High dose group,with high fatsugar diets and 45 mg/kg SZT) developed diabetic symptoms,its FBG was above the required level,while Metformin Hydrochloride(200 mg/kg),Glimepiride(2 mg/kg)could indeed significantly bring their sugar levels down.Conclusion The method of inducing type 2 rat diabetes by high-fat feeding combined with STZ is effective and the models are stable for a long period of time.