Morphological Characteristics of Phanerochaete chrysosporium in a Reactor Operated under Non-Sterile Conditions

To clarify the morphological characteristics of white-rot fungi is helpful for achieving the continuous operation of white-rot fungal reactor. The white-rot fungal reactor coupled with an ozone unit was operated continually to degrade acid blue 45. The morphological changes of immobilized and non-immobilized white-rot fungi was investigated. The results showed that contaminating microorganisms can be controlled at 7. 1 × 105CFU/mL and the average decolorization of acid blue 45 achieved 19% in the non-immobilized mycelium system inoculated with1700 mg/L P. chrysosporium,but the pellet of P. chrysosporium were unstable,the hyphal fragments fallen-off from the pellet surface after8-12 days operation and some pellets ruptured. The cell contents outflew from those ruptured hyphae,by which contaminating microorganisms were nourished. In contrast,the systems inoculated with 1700 mg/L immobilized mycelium of P. chrysosporium have low numbers of contaminating bacteria( 8. 4 × 104CFU/mL) and a high dye removal rate( 22%); Contaminating bacteria can be controlled at 4. 7 × 104CFU/mL,the average decolorization achieved 29% in the immobilized mycelium system inoculated with 4300 mg/L immobilized mycelium of P. chrysosporium. In the immobilized mycelium system,some hyphal fragments also fallen-off from the immobilized mycelium surface after21 days reactor operation. The hyphal fragments fallen-off from the pellets or immobilized mycelium correlated with the aging and autolysis of hyphal that could interfere with the continuous grow of white-rot fungi,which may be solved by using novel carrier by which nutrient substance and oxygen can be supplied from the internal and external of the attached biofilms to promoted the continuous growth of white-rot fungi.