亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Decreased glycolate oxidase activity leads to altered carbon allocation and leaf senescence after a transfer from high CO2to ambient air inArabidopsis thaliana

光呼吸 拟南芥 光合作用 鲁比斯科 叶绿素荧光 生物化学 拟南芥 新陈代谢 叶绿素 选择性氧化酶 化学 生物 突变体 植物 基因
作者
Younès Dellero,Mathieu Jossier,Nathalie Glab,Céline Oury,Guillaume Tcherkez,Michael Hodges
出处
期刊:Journal of Experimental Botany [Oxford University Press]
卷期号:67 (10): 3149-3163 被引量:35
标识
DOI:10.1093/jxb/erw054
摘要

Metabolic and physiological analyses of Arabidopsis thaliana glycolate oxidase (GOX) mutant leaves were performed to understand the development of the photorespiratory phenotype after transfer from high CO2 to air. We show that two Arabidopsis genes, GOX1 and GOX2, share a redundant photorespiratory role. Air-grown single gox1 and gox2 mutants grew normally and no significant differences in leaf metabolic levels and photosynthetic activities were found when compared with wild-type plants. To study the impact of a highly reduced GOX activity on plant metabolism, both GOX1 and GOX2 expression was knocked-down using an artificial miRNA strategy. Air-grown amiRgox1/2 plants with a residual 5% GOX activity exhibited a severe growth phenotype. When high-CO2-grown adult plants were transferred to air, the photosynthetic activity of amiRgox1/2 was rapidly reduced to 50% of control levels, and a high non-photochemical chlorophyll fluorescence quenching was maintained. (13)C-labeling revealed that daily assimilated carbon accumulated in glycolate, leading to reduced carbon allocation to sugars, organic acids, and amino acids. Such changes were not always mirrored in leaf total metabolite levels, since many soluble amino acids increased after transfer, while total soluble protein, RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase), and chlorophyll amounts decreased in amiRgox1/2 plants. The senescence marker, SAG12, was induced only in amiRgox1/2 rosettes after transfer to air. The expression of maize photorespiratory GOX in amiRgox1/2 abolished all observed phenotypes. The results indicate that the inhibition of the photorespiratory cycle negatively impacts photosynthesis, alters carbon allocation, and leads to early senescence in old rosette leaves.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
丘比特应助科研通管家采纳,获得10
38秒前
1分钟前
小秦同学发布了新的文献求助10
1分钟前
小秦同学完成签到,获得积分20
1分钟前
JcZuk完成签到,获得积分10
1分钟前
1分钟前
1分钟前
SOLOMON应助fandan采纳,获得10
1分钟前
愉快谷芹完成签到 ,获得积分10
2分钟前
tan发布了新的文献求助10
2分钟前
隐形曼青应助科研通管家采纳,获得10
2分钟前
3分钟前
小秦同学发布了新的文献求助30
3分钟前
tan完成签到 ,获得积分10
3分钟前
3分钟前
Wilson完成签到 ,获得积分10
4分钟前
SOLOMON应助科研通管家采纳,获得10
4分钟前
4分钟前
neuroman完成签到 ,获得积分10
5分钟前
忧虑的安青完成签到,获得积分10
6分钟前
吃鱼完成签到 ,获得积分10
6分钟前
6分钟前
SOLOMON应助科研通管家采纳,获得10
6分钟前
大模型应助Wei采纳,获得10
6分钟前
6分钟前
Zoe先生完成签到,获得积分10
7分钟前
8分钟前
LST发布了新的文献求助10
8分钟前
SOLOMON应助科研通管家采纳,获得10
8分钟前
Wei发布了新的文献求助10
8分钟前
8分钟前
wshwx完成签到 ,获得积分10
9分钟前
9分钟前
cytb6f发布了新的文献求助10
9分钟前
9分钟前
烟花应助ektyz采纳,获得20
9分钟前
cytb6f完成签到,获得积分10
9分钟前
9分钟前
ektyz发布了新的文献求助20
10分钟前
11分钟前
高分求助中
请在求助之前详细阅读求助说明!!!! 20000
One Man Talking: Selected Essays of Shao Xunmei, 1929–1939 1000
The Three Stars Each: The Astrolabes and Related Texts 900
Yuwu Song, Biographical Dictionary of the People's Republic of China 800
Multifunctional Agriculture, A New Paradigm for European Agriculture and Rural Development 600
Bernd Ziesemer - Maos deutscher Topagent: Wie China die Bundesrepublik eroberte 500
A radiographic standard of reference for the growing knee 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2478133
求助须知:如何正确求助?哪些是违规求助? 2141384
关于积分的说明 5458861
捐赠科研通 1864637
什么是DOI,文献DOI怎么找? 926957
版权声明 562897
科研通“疑难数据库(出版商)”最低求助积分说明 496007