卵巢
生物
输卵管
间质细胞
转录组
电池类型
中国仓鼠卵巢细胞
细胞
染色质
细胞生物学
免疫系统
细胞培养
基因
癌症研究
基因表达
免疫学
遗传学
解剖
作者
Ernst Lengyel,Yan Li,Melanie Weigert,Lisha Zhu,Heather Eckart,Melissa Javellana,Sarah Ackroyd,Jason Xiao,Susan Olalekan,Dianne Glass,Shilpa Iyer,Agnes Bilecz,Ricardo R. Lastra,Mengjie Chen,Anindita Basu
标识
DOI:10.1101/2022.08.04.502826
摘要
Summary As part of the Human Cell Atlas initiative, we generated transcriptomic (scRNA-seq; 86,708 cells) and regulatory (scATAC-seq; 59,118 cells) profiles of the normal postmenopausal ovary and fallopian tube (FT) at single-cell resolution. In the FT, 22 cell clusters integrated into 11 cell types, including ciliated and secretory epithelial cells, while the ovary had 17 distinct cell clusters defining 6 major cell types. The dominant cell type in both the postmenopausal ovary and FT was stromal cells, which expressed several genes associated with aging. The fimbrial end of the FT had a significant number and variety of immune cells and active communication with the ovary; the ovary contained mostly stromal cells but few immune cells. The epithelial cells of the normal FT expressed multiple ovarian cancer risk-associated genes ( CCDC170, RND3, TACC2, STK33 , and ADGB ). By integrating paired single-cell transcriptomics and chromatin accessibility data we found that the regulatory landscape of the fimbriae was markedly different from the isthmus and ampulla. Intriguingly, several cell types in the FT had comparable gene expression but different transcriptional regulations. Our single-cell transcriptional and regulatory maps allowed us to disentangle the complex cellular makeup of the postmenopausal FT and ovary and will contribute to a better understanding of gynecologic diseases in menopause.
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