Histological effects of a combined collagen stimulation procedure consisting of microfocused ultrasound, soft tissue filler, and Ca‐HA injections

Abstract Background An increasing demand for in‐office aesthetic procedures aiming to improve collagen stimulation has been reported. Objective To analyze the effects of combinations of different aesthetic collagen stimulation procedures using histological analysis. Methods Histological analysis was performed on skin samples of the excess skin of a 60‐year‐old patient after sub‐superficial musculo‐aponeurotic system facelift surgery. The excess skin before facelift surgery was divided into three areas per hemiface. Each area—with exception of area A as a control—received an isolated or combined treatment of microfocused ultrasound (MFU), injections of calcium‐hydroxylapatite/hyaluronic‐acid‐based soft tissue filler, and microneedling. The effect of the treatments was analyzed using histological analysis with H&E coloration and PAS staining. Results A threefold increase of epidermal and dermal thickness was achieved by treating the skin with the proposed combined treatment comprising MFU and injections of calcium‐hydroxylapatite‐ and hyaluronic‐acid‐based soft tissue fillers. Conclusion The investigated treatments act synergistically in the production of collagen and combining these treatments allows for an increase in collagen production.