荧光计
分析物
核黄素
校准
检出限
RGB颜色模型
探测器
像素
计算机科学
数字图像
校准曲线
光电二极管
信号(编程语言)
荧光
材料科学
生物系统
光学
化学
人工智能
图像处理
色谱法
数学
光电子学
图像(数学)
物理
统计
生物化学
生物
程序设计语言
作者
Diana Pereira,Mariana Mourão Matos,Adelaida Pallavicini Fonseca
出处
期刊:Analytical Methods
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:15 (37): 4954-4963
摘要
This work describes the construction and evaluation of a fluorometer for use in paper analytical devices, using a smartphone to operate the instrument and to perform real-time image-based detection. In this approach, a circular PAD containing twenty analytical plates is rotated at 18° increments under a UV LED source, providing a sequential irradiation of plates and the detection of the luminescence with a lab-made application, capable of automatically identifying the analytical zones and collecting the RGB intensities from the selected pixels. As a proof of concept, the fluorometer performance was evaluated for the determination of quinine in beverages and riboflavin (B2 vitamin) in supplements. Quinine, which is less photoreactive, provided steady-state signals, while riboflavin, which rapidly degrades under UV photons, presented transient responses for RGB detection. For both analytes, linear calibration ranges (R2 > 0.99) were observed from 2.0 mg L-1 to 10.0 mg L-1 with limits of detection estimated at approximately 1.0 mg L-1. Nevertheless, it was demonstrated that successive additions of standard solutions to a single analytical plate of PAD could enhance the signal-to-noise ratios for less concentrated samples, acting as a pre-concentration step. In addition, suitable deviations for the signals (ca. 4.0%) and the absence of systematic errors for most samples (9 out of 11), when compared with a reference method at 95% confidence level, indicates that the proposed strategy is precise and accurate enough to be used as analytical tool for fluorescence detection in PAD.
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