肌萎缩
骨骼肌
蛋白质组学
质谱法
生物
计算生物学
生物化学
化学
解剖
色谱法
基因
作者
Melissa R Pergande,Katie Osterbauer,Kevin M. Buck,David S. Roberts,Nigel B. Wood,Priya Balasubramanian,Morgan Mann,Kalina J. Rossler,Gary M. Diffee,Ricki J. Colman,Rozalyn M. Anderson,Ying Ge
标识
DOI:10.1021/acs.jproteome.3c00474
摘要
Sarcopenia is a progressive disorder characterized by age-related loss of skeletal muscle mass and function. Although significant progress has been made over the years to identify the molecular determinants of sarcopenia, the precise mechanisms underlying the age-related loss of contractile function remains unclear. Advances in "omics" technologies, including mass spectrometry-based proteomic and metabolomic analyses, offer great opportunities to better understand sarcopenia. Herein, we performed mass spectrometry-based analyses of the vastus lateralis from young, middle-aged, and older rhesus monkeys to identify molecular signatures of sarcopenia. In our proteomic analysis, we identified proteins that change with age, including those involved in adenosine triphosphate and adenosine monophosphate metabolism as well as fatty acid beta oxidation. In our untargeted metabolomic analysis, we identified metabolites that changed with age largely related to energy metabolism including fatty acid beta oxidation. Pathway analysis of age-responsive proteins and metabolites revealed changes in muscle structure and contraction as well as lipid, carbohydrate, and purine metabolism. Together, this study discovers new metabolic signatures and offers new insights into the molecular mechanisms underlying sarcopenia for the evaluation and monitoring of a therapeutic treatment of sarcopenia.
科研通智能强力驱动
Strongly Powered by AbleSci AI