化学
适体
细胞外小泡
糖基
印记(心理学)
分子印迹
小泡
发光测量
生物化学
色谱法
生物物理学
分子生物学
发光
细胞生物学
基因
选择性
物理
光电子学
膜
生物
催化作用
作者
Zejun Jiang,Kui Luo,Guangwei Yang,Yang Li,Ling Li,Guocong Wang,Tao Qin,Jianping Li
标识
DOI:10.1021/acs.analchem.3c04991
摘要
Cancer-related extracellular vesicles (EVs) are considered important biomarkers for cancer diagnosis because they can convey a large amount of information about tumor cells. In order to detect cancer-related EVs efficiently, an electrochemiluminescence (ECL) sensor for the specific identification and highly sensitive detection of EVs in the plasma of cancer patients was constructed based on dual recognitions by glycosyl-imprinted polymer (GIP) and aptamer. The characteristic glycosyl Neu5Ac-α-(2,6)-Gal-β-(1–4)-GlcNAc trisaccharide on the surface of EVs was used as a template molecule and 3-aminophenylboronic acid as a functional monomer to form a glycosyl-imprinted polymer by electropolymerization. After glycosyl elution, the imprinted film specifically recognized and adsorbed the EVs in the sample, and then the CD63 aptamer–bipyridine ruthenium (Aptamer–Ru(bpy)) was added to combine with the CD63 glycoprotein on the extracellular vesicle’s surface, thus providing secondary recognition of the EVs. Finally, the EVs were quantitatively detected according to the ECL signal produced by the labeled bipyridine ruthenium. When more EVs were captured by the imprinted film, more probes were obtained after incubation, and the ECL signal was stronger. Under the optimized conditions, the ECL signal showed a good linear relationship with the concentration of EVs in the range of 9.5 × 102 to 9.5 × 107 particles/mL, and the limit of detection was 641 particles/mL. The GIP sensor can discriminate between the EV contents of cancer patients and healthy controls with high accuracy. Because of its affordability, high sensitivity, and ease of use, it is anticipated to be employed for cancer early detection and diagnosis.
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