Green microextraction techniques in therapeutic drug monitoring

Therapeutic drug monitoring (TDM) is crucial for optimizing drug therapy, particularly for medications with narrow therapeutic windows. By measuring drug concentrations in biological fluids, TDM aids in dose individualization, adherence assessment, and the prevention of adverse effects. Recent advancements focus on miniaturized and eco-friendly sample preparation techniques, such as microextraction methods, which are efficient and low-cost. These include liquid- and solid-phase microextraction, offering benefits such as automation and high-throughput performance which have significantly improved TDM efficiency. Additionally, the emergence of microsampling offers a less invasive alternative to traditional blood collection for TDM and pharmacokinetic studies. Coupling these innovations with sensitive analytical techniques, like liquid chromatography-tandem mass spectrometry, enables accurate and reliable drug quantification from minimal sample volumes. These developments collectively enhance the clinical utility of TDM and contribute to improved patient outcomes. This paper provides a comprehensive review of recent advancements in green microextraction techniques as applied to therapeutic drug monitoring.