Helicobacter pylori Virulence Factor Genotyping

Helicobacter pylori (H. pylori) Helicobacter pylori (H. pylori) infection Infections causes chronic gastritisGastritis, peptic ulcersPeptic ulcers, gastric adenocarcinoma, and mucosa-associated lymphoid tissue (MALT) lymphoma. Bacterial, host, and environmental factors influence the progression of disease from superficial gastritisGastritis to cancer. H. pylori is genetically diverse, and expression of its specific virulence factorsVirulence factors has been linked to increased risk of more severe pathologies. Described in this chapter is a protocol for detecting important H. pylori virulence factorsVirulence factors by firstly extracting DNA from culture material or stomach tissue biopsies, followed by PCR amplificationPCR Amplifications and agarose gel electrophoresisAgarose gel electrophoresis.