Estrogen Receptor β-Targeted Near-Infrared Inherently Fluorescent Probe: A Potent Tool for Estrogen Receptor β Research

Estrogen receptor β (ERβ) is associated with many diseases, and ERβ probes can help to reveal the complex role of ERβ and promote the development of ERβ-targeted therapy. Herein, we designed and synthesized the first ERβ-targeted near-infrared (NIR) inherently fluorescent probe P5, which showed the advantages of high ERβ selectivity, good optical properties, and excellent ERβ imaging capability in living cells. The probe was successfully utilized to explore ERβ motion characteristic, and for the first time, the diffusion coefficient of ERβ was obtained. Moreover, P5 was also successfully applied to the in vivo imaging of ERβ in the prostate cancer mice model. Therefore, this ERβ-targeted NIR probe might be employed as a potential tool for the research of ERβ and related diseases.