Structural characterization and anti-inflammatory properties of green synthesized chitosan/compound K‑gold nanoparticles

Ginsenoside compound K (CK) has been shown to exhibit anti-inflammatory properties. In this study, to encourage biomedical applications of biosynthesized gold nanoparticles (AuNPs) with anti-inflammatory effects, AuNPs loaded with ginsenoside compound K were prepared using a self-assembly technique with chitosan as the carrier. Optimal conditions for chitosan-ginsenoside CK‑gold nanoparticles (CS-CK-AuNPs) formation were monitored using UV-Vis absorption spectroscopy. The physicochemical characterization of CS-CK-AuNPs was performed using FE-TEM, FE-SEM, XRD, DLS, FTIR and NMR techniques. In the stability test, CS-CK-AuNPs did not show any significant changes up to 4 weeks. Fluorescence imaging demonstrated that CS-CK-AuNPs promoted cellular uptake in vitro, but did not exhibit significant cytotoxicity at concentrations below 40 μg/mL. Additionally, the CS-CK-AuNPs inhibited NO production, and reduced the expression and secretion of inflammatory cytokines (IL-1β, IL-6, and TNF-α) via inhibition of the nuclear factor-kappaB (NF-κB) pathway in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Thus, CS-CK-AuNPs are novel candidates for developing anti-inflammatory agent. This study also confirms the superiority of chitosan AuNPs as oral delivery vehicles for inflammation-related diseases.