MP19-04 IN-VIVO REGENERATION OF BLADDER MUSCULAR WALL WITH DECELLULARIZED COLON MATRIX: AN EXPERIMENTAL STUDY

You have accessJournal of UrologyStem Cell Research1 Apr 2015MP19-04 IN-VIVO REGENERATION OF BLADDER MUSCULAR WALL WITH DECELLULARIZED COLON MATRIX: AN EXPERIMENTAL STUDY Abdol-Mohammad Kajbafzadeh, Reza Khorramirouz, Shabnam Sabetkish, Nastaran Sabetkish, Ahmad Masoumi, and Sorena Keihani Abdol-Mohammad KajbafzadehAbdol-Mohammad Kajbafzadeh More articles by this author , Reza KhorramirouzReza Khorramirouz More articles by this author , Shabnam SabetkishShabnam Sabetkish More articles by this author , Nastaran SabetkishNastaran Sabetkish More articles by this author , Ahmad MasoumiAhmad Masoumi More articles by this author , and Sorena KeihaniSorena Keihani More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.996AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Bladder wall regeneration in pediatrics remains a serious challenge for surgeons. This study aims to determine histological aspects and biocompatibility of decellularized colon graft for bladder augmentation in a rat model. METHODS The rat colon tissues were decellularized using perfusion-based method and underwent different laboratory investigations to determine the efficacy of decellularization in preserving the extracellular matrix (ECM) scaffold and cell removal. After partial cystectomy (approximately 35–40%) in twelve rats, the bladder was grafted with a patch of decellularized colon (approximately 1×2 cm2). Then, the bladder was filled with normal saline to observe probable leakage from the suture sites. Bladder biopsies were taken at 1, 3, and 9 months postoperatively for further histological investigations. RESULTS Total cell removal with intact ECM structure and without collagen degradation was confirmed in decellularized colon. The villus-crypt architecture was preserved in the scaffold lumen. All bladders demonstrated a spherical shape without any necrosis, urinary leakage, or graft extrusion in any of the animals. One month after implantation, partial seeding of new cells was observed. After 3 months of operation, the region of the graft was indistinguishable from natural bladder and continuity of transitional epithelium of natural bladder on the decellularized grafted colon tissue was confirmed with histological examination. The presence of urothelium with similar morphology to the natural urothelium and visible blood vessels were found within 3 months of operation. CD34 endothelial progenitor cells, CD31 microvessels, α-smooth muscle actin (α-SMA), S100, cytokeratin AE1/AE3, vimentin, and synaptophysin were expressed highly in immunohistochemical staining after 9 months of operation. In addition, the uniform cylindrical shape of decellularized colon was changed to transitional form after implantation. CONCLUSIONS The results of the current study suggest that decellularized colon is biocompatible and can be considered as a reliable natural collagen scaffold and viable material for bladder augmentation. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e216 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Abdol-Mohammad Kajbafzadeh More articles by this author Reza Khorramirouz More articles by this author Shabnam Sabetkish More articles by this author Nastaran Sabetkish More articles by this author Ahmad Masoumi More articles by this author Sorena Keihani More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...