Objective To compare the results of glucose challenge test and oral glucose tolerance test.in diagnosis of gestational diabetes mellitus. Methods The informed content glucose challenge test and oral glucose tolerance test were carried out in voluntary pregnant women after positive screening and the results were analyzed. Results Glucose challenge test was conducted on 681 pregnant women and 78 of them were positive for gestational diabetes mellitus with a positive rate of 11.45%; then oral glucose tolerance test was performed in the 78 positives and 65 of them were positive for gestational diabetes mellitus with the positive rate of 9.54% ( 83.33%),without showing statistically significant differences between the two tests(χ2=1.32,P=0.25,P0.05). Conclusions The detection rate of glucose challenge test in diagnosis of gestational diabetes mellitus is high and it is simple and practicable for clinical purpose.