Использование молекулярных методов для видовой идентификации пробиотических лактобактерий

Comparative analysis of nucleotide sequences of three strains of lactic acid bacteria with reference strains deposited in the NCBI database. Identified to the species of lactic acid bacteria, which are antagonists against pathogenic and conditionally pathogenic bacteria. The results of the analysis of the nucleotide sequence of a fragment of 16S rRNA showed that the studied strains assigned to the genus Lactobacillus. Phylogenetic analysis of a fragment of 16S rRNA revealed that the strains No. 27, No. 27W and No. 18g form one cluster with strains of Lactobacillus farraginis, Lactobacillus faeni and Lactobacillus zeae, Lactobacillus paracasei and Lactobacillus casei belong to the phylogenetic qroup of Lactobacillus buchneri and Lactobacillus casei/paracasei.