Soy protein isolate supplemented silk fibroin nanofibers for skin tissue regeneration: Fabrication and characterization

Abstract Biocompatible soy protein isolate/silk fibroin (SPI/SF) nanofibrous scaffolds were successfully fabricated through electrospinning a novel protein blend SPI/SF. Prepared nanofibers were treated with ethanol vapor to obtain an improved water-stable structure. Fabricated scaffolds were characterized through scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), UV–VIS spectrophotometry and image analysis. The mean diameters of SPI/SF electrospun fibers were observed ranging between 71 and 160 nm. The scaffolds were found significantly stable for a prolong duration at the room temperature as well as at 37 °C, when placed in phosphate buffered saline, nutrient medium, and lysozyme-containing solution. The potential of fabricated scaffolds for skin tissue regeneration was evaluated by in vitro culturing of standard cell lines i.e., fibroblast cells (L929-RFP (red fluorescent protein) and NIH-3T3) and melanocytes (B16F10). The outcomes revealed that all the fabricated nanofibrous scaffolds were non-toxic towards normal mammalian cells. In addition, healing of full-thickness wound in rats within 14 days after treatment with a nanofibrous scaffold demonstrated its suitability as a potential wound dressing material. Interestingly, we found that nanofibers induced a noticeable reduction in the proliferation rate of B16F10 melanoma cells.