Reversible Enzyme Inhibition–Based Biosensors: Applications and Analytical Improvement Through Diagnostic Inhibition

Abstract The present review reports the research carried out during past 9 years on biosensors based on reversible enzyme inhibition for determination of drugs, pollutants, and toxic compounds. Applications in food, environmental, and pharmaceutical fields are also reported. Special attention is paid to the optimization of parameters such as enzyme immobilization, substrate concentration, and incubation time. On the basis of the studies reviewed here, it is our view that enzyme inhibition–based biosensors have been shown to be useful analytical tools.