丙二醛
化学
硫代巴比妥酸
色谱法
脂质过氧化
高效液相色谱法
水解
氨基酸
生物化学
抗氧化剂
作者
H. H. Draper,E. J. Squires,Haleh Mahmoodi,Juyou Wu,Sanjiv Agarwal,M. Hadley
标识
DOI:10.1016/0891-5849(93)90035-s
摘要
A comparative evaluation was made of the conventional spectrophotometric procedure and three published high performance liquid chromatographic (HPLC) procedures for the determination of malondialdehyde (MDA) as the thiobarbituric acid (TBA) derivative when applied to liver, fish meal, serum, and urine. Except for urine, spectrophotometric analysis overestimated MDA content. Purification of the TBA-MDA complex obtained from liver and fish meal on reverse phase cartridges was found to entail a loss of complex bound to residual peptides in the trichloracetic acid (TCA) extract. Mincing as opposed to homogenizing liver samples led to a doubling of values for MDA content. Hexanal was a major TBA reactant, in addition to MDA, in all the samples. Acid hydrolysis and heat were necessary for the release of MDA bound to the amino groups of proteins and other amino compounds. Methods for free MDA have limited application to biological materials except short term in vitro preparations such as peroxidizing microsomes, in which free MDA accumulates. On the basis of these and other observations, a modified HPLC procedure for the determination of MDA as the TBA-MDA complex is proposed.
科研通智能强力驱动
Strongly Powered by AbleSci AI