基因剔除小鼠
子宫
生物
基因敲除
卵巢
Cre重组酶
内科学
内分泌学
雌激素受体
生殖系统
不育
核受体
雌激素
生殖道
野生型
受体
细胞生物学
基因
转基因
转基因小鼠
遗传学
医学
突变体
癌症
乳腺癌
转录因子
作者
Per Antonson,Yoko Omoto,Patricia Humire,Jan‐Åke Gustafsson
标识
DOI:10.1016/j.bbrc.2012.07.016
摘要
Estrogen receptor alpha (ERα) is a nuclear receptor that regulates a range of physiological processes in response to estrogens. In order to study its biological role, we generated a floxed ERα mouse line that can be used to knock out ERα in selected tissues by using the Cre/LoxP system. In this study, we established a new ERα knockout mouse line by crossing the floxed ERα mice with Cre deleter mice. Here we show that genetic disruption of the ERα gene in all tissues results in sterility in both male and female mice. Histological examination of uterus and ovaries revealed a dramatically atrophic uterus and hemorrhagic cysts in the ovary. These results suggest that infertility in female mice is the result of functional defects of the reproductive tract. Moreover, female knockout mice are hyperglycemic, develop obesity and at the age of 4 months the body weight of these mice was more than 20% higher compared to wild type littermates and this difference increased over time. Our results demonstrate that ERα is necessary for reproductive tract development and has important functions as a regulator of metabolism in females.
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