Chlorogenic Acid and Rutin content comparison in different processed Ilex hainanensis Merr.

Objective To explore the Changing regularity of Chlorogenic acid and Rutin content in different processed Ilex hainanensis Merr.was studied by HPLC.In order to decide the best processed methods.Methods Using RP-HPLC to determine the contents of Chlorogenic acid and Rutin in different processed Ilex hainanensis Merr..Results Chlorogenic acid peak area in the sample volume was a good linear relationship between 0.10 ~ 2.00μg.The average recovery of 97.18%,RSD was1.27%,tradition processing methods have highest Chlorogenic acid concentrations.The results also show that Rutin peak area in the sample volume was a good linear relationship between 0.25 ~ 5.00μg.The average recovery of 99.36%,RSD was1.91%,tradition processing methods have highest Rutin concentrations.Conclusion Establish a methods for HPLC content determination methods can be use for quality control of different processed of Ilex hainanensis Merr..Tradition processing Ilex hainanensis Merr.has highest Chlorogenic acid and Rutin concentrations.