In Vitro Selection of DNA-cleaving DNAzyme

In order to obtain single-standed-DNA-cleaving artificial DNA enzyme,we carried out in vitro selection and got the DNAzyme F-9 that consists of a catalytic domain flanked by two single-strand binging arms.F-9 DNA enzyme can cleave DNA in a certain concentration of copper and manganese metal ions.F-9 can cleave DNA sequence containing ACTG.Study of the splice site of DNA substrate found that F-9 undergoes cleavage at several closely-grouped sites.