Clone and sequence analysis of GABA receptor 5' fragment from Bactrocera dorsalis strain resistant to avermectin

Resistant strains of oriental fruit fly Bactrocera dorsalis( Hendel) to the avermectin was developed,and the resistance fold was 113. 12. Gene of encoding GABA receptor of susceptible strain and AvR90were cloned by PCR. A fragment of 1053 bp was amplified in susceptible strain and AvR90 strain. The DNA sequences between susceptible strain and AvR90strain had one difference( A496T), but the amino acid sequences from the fragment were identical.