Study on apoptosis of human cervical cancer HeLa cell induced by Ilexgenin A and its primary mechanism

Objective: To investigate the apoptosis of human cervical cancer HeLa cell induced by Ilexgenin A(IA) and its primary mechanism. Methods: The viability of HeLa cells was detected by the method CCK-8. The activity of LDH was measured by MTT assay. Immune colorimetry was used to test the proliferation of Hela cells. The change of morphology of apoptosis HeLa cells was observed by DAPI l uorescence staining. Western Blot was used to detect the expressions of Caspase-3/7 and NF-κB proteins of HeLa cells. Results: After HeLa cells were treated by IA, the leakage of LDH increased in a dosage and time dependent manner. In the process of determination of BrdU and CCK-8, the inhibitory ef ect of IA to HeLa cell was stronger than standard drugs, and showed clear inhibitory ef ect on the HeLa cell proliferation with the increasing of concentration and the acting time. By using the DAPI l uorescence dyes, the HeLa cell cytoplasm concentrating, nucleolus dispersion and chromatin aglomeration were observed. Western Blot analysis showed that the expression of Caspase-3/7 was significantly enhanced and the expression of NF-κB proteins was down-regulated. Conclusion: IA could induce the apoptosis of HeLa cells and its mechanism is related to activate the activity of Caspase-3/7 and down-regulate the expression of NF-κB protein.