Anti-tumor Effect of Cooperation of Cinobufacine Injection with Mitomycin C in vitro

Objective:To investigate the anti tumor effects of cooperation of cinobufacine injection with mitomycin C in vitro.Method: The cytotoxicity of mitomycin C cooperated with cinobufacine injection against established hepato cell lines(SMMC 7721,BEL 7402 and L 02 cell)was determined by MTT assays. Mitomycin C, cinobufacine injection were used separately as control. Result: Cooperation of cinobufacine injection with mitomycin C showed much more better anti tumor effect than the drugs used separately, but do not significantly enhance the growth inhibitory rate of normal hepatic cell lines. Conclusion: Cooperation of cinobufacine injection with mitomycin C has better anti tumor effect than the drugs used separately,meanwhile it do not enhance cytotoxicity to normal hepatic cell line, showing its feasibility and validity in hepatocellular carcinoma curing.