[Objective] The purpose was to observe the effects of He-Ne laser on the conductivity of nutrient solution and distilled water so as to provide new information for discussing the effect and action mechanism of laser.[Method] The nutrient solution and distilled water were irradiated with He-Ne laser at special intensity for different durations.[Result] Compared with the non-irradiated nutrient solution,except for irradiation for 4 min,the conductivity of irradiated nutrient solution was increased significantly and its variation had no correlation with irradiation duration.As the irradiation duration extended,the conductivity of distilled water was first decreased and then increased.When the irradiation duration was shorter than 10 min,the conductivity of irradiated distilled water was extremely significantly lower than that of non-irradiated distilled water.When the irradiation duration was longer than 10 min,the conductivity of irradiated distilled water was extremely significantly higher than that of non-irradiated distilled water.When the irradiation duration was 15 min,the conductivity of irradiated distilled water was highest.[Conclusion] The laser at certain frequence and intensity could induce the variation of conductivity of nutrient solution and distilled water.