Research on preparation of β-carotene nanoemulsions with different emulsifiers

β-carotene nanoemulsions were prepared through high pressure homogenization,with four different emulsifiers,Tween 20(TW),decaglycerol monolaurate(DML),octenyl succinate starch(OSS) and whey protein isolate(WPI).Droplet size was analysed by Zetasizer,and nanoemulsion stability was detected through Turbiscan.HPLC was applied to determine the content of β-carotene.Results indicated that nanoemulsions had the droplet size of 115.0～303.0nm and polydispersity of 0.09～0.30,and droplets in nanoemulsions prepared with TW,DML were much smaller than those with OSS and WPI(p0.05).During storage,droplet size significantly increased at 55℃(p0.05),but remained stable at 4℃.β-carotene in OSS stabilized emulsion degraded quickly,only 22.88% and 26.23% being kept when stored at 55℃ for 12d and 4℃ for 28d.While WPI showed good capacity to protect β-carotene,72.23% and 62.08% being kept under the same storage conditions.